An Ultrasensitive Bioluminescent Enzyme Immunoassay Based on Nanobody/Nanoluciferase Heptamer Fusion for the Detection of Tetrabromobisphenol A in Sediment
Zhenfeng Li, Yi Wang, Natalia Vasylieva, Debin Wan, Zihan Yin, Jie-Xian Dong, Bruce D. Hammock
Abstract
of 28.9 pg/mL and the limit of detection as low as 2.5 pg/mL. The proposed assay was further applied to determine the trace TBBPA in sediment, and the recovery was within 92-103%. Taking advantage of this heptamer fusion, one-step BLEIA can serve as a powerful tool for fast detection of trace TBBPA in the sediment samples.
Topics & Concepts
Tetrabromobisphenol AChemistryImmunoassayDetection limitChromatographyReagentFire retardantAntibodyBiologyOrganic chemistryPhysical chemistryImmunologyPharmaceutical and Antibiotic Environmental ImpactsAnalytical chemistry methods developmentAdvanced biosensing and bioanalysis techniques