Water as a Structural Marker in Gelatin Hydrogels with Different Cross-Linking Nature
Yuriy F. Zuev, Svetlana R. Derkach, I. V. Lunev, Alena Nikiforova, Mariya A. Klimovitskaya, L. R. Bogdanova, Polina V. Skvortsova, Rauf Kurbanov, Mariia A. Kazantseva, О. С. Зуева
Abstract
We have studied the molecular properties of water in physically and chemically cross-linked gelatin hydrogels by FTIR-spectroscopy, NMR relaxation, and diffusivity and broadband dielectric spectroscopy, which are sensitive to dynamical properties of water, being a structural marker of polymer network. All experiments demonstrated definite reinforcement of the hydrogel net structure and an increase in the amount of hydrate water. FTIR experiments have shown that the chemical cross-linking of gelatin molecules initiates an increase in the collagen-like triple helices "strength", as a result of infused restriction on protein molecular mobility. The "strengthening" of protein chains hinders the mobility of protein fragments, introducing complex modifications into the structural properties of water which are remained practically unchanged up to up to 30-40 °C.