Fine-Tuning of Transcription in <i>Pichia pastoris</i> Using dCas9 and RNA Scaffolds
Michael Baumschabl, Roland Prielhofer, Diethard Mattanovich, Matthias G. Steiger
Abstract
overexpression with a pGAP promoter.
Topics & Concepts
Pichia pastorisYeastActivator (genetics)Cas9GenePsychological repressionTranscription (linguistics)PromoterSynthetic biologyPichiaGene expressionBiologyMetabolic engineeringRNAComputational biologyCRISPRCell biologyGeneticsRecombinant DNALinguisticsPhilosophyCRISPR and Genetic EngineeringMicrobial Metabolic Engineering and BioproductionPhotosynthetic Processes and Mechanisms