Rhodococcus pseudokoreensis sp. nov. isolated from the rhizosphere of young M26 apple rootstocks
Peter Kämpfer, Stefanie P. Glaeser, Jochen Blom, Jacqueline Wolf, Sarah Benning, Michael Schloter, Meina Neumann‐Schaal
Abstract
Abstract The Gram-positive strain R79 T , isolated from the rhizosphere of young M26 apple rootstocks, was investigated by a polyphasic taxonomic approach. Phylogenetic identification based on the full-length 16S rRNA gene sequence revealed highest 16S rRNA gene sequence similarity to the type strains of Rhodococcus wratislaviensis (99.6%) and Rhodococcus opacus (99.2%) followed by Rhodococcus imtechensis (98.9%). All other 16S rRNA gene sequence similarities were below 98.65%. A phylogenomic tree calculated based on a whole-genome sequence also showed a distinct clustering with the type strain of Rhodococcus koreensis. Average nucleotide identity (ANI) values between whole-genome sequences of R79 T and the closest related type strains were below 95% supported the novel species status. The DNA G + C content of R79 T was 67.24% mol. Predominant fatty acids were C 16:0, C 15:0 and C 17:1ω8c . The strain contained MK8-H 2 as the major respiratory quinone. The polar lipid profile consists of diphosphatidylglycerol and phosphatidylethanolamine, as well as of some unidentified lipids. The peptidoglycan type of the strain is A1γ meso -diaminopimelic acid. Based on the obtained genotypic and phenotypic, including chemotaxonomic data, we conclude that R79 T represents a novel species of the genus Rhodococcus , for which the name Rhodococcus pseudokoreensis sp. nov. is proposed. The type strain is R79 T (= DSM 113102 T = LMG 32444 T = CCM 9183 T ).