In Vitro Comparison of Antibacterial Effect of Ozonated Water and Ozonated Gas
Felipe Agostini, Melissa Faccini, Francisco Fitarelli, Mariana Aparecida Lopes Ortiz, Samira Salmeron, Ricardo César Gobbi de Oliveira, Fabrício Pinelli Valarelli, Renata Cristina Gobbi de Oliveira, Karina Maria Salvatore de Freitas
Abstract
This study aimed to compare the antibacterial effect of ozonated water and ozonated gas at different concentrations and exposure times. Staphylococcus aureus and Enterococcus faecalis were exposed to ozonated water or ozonated gas at concentrations of 20, 40 and 60 μg/mL for 1 and 2 minutes. A positive control with bacteria and a chlorhexidine 2% negative control were used. The number of colony-forming units (CFU/mL) was evaluated. The concentrations of 40 and 60 μg/mL were significantly more effective. For the E. faecalis, ozonated gas was significantly more effective than ozonated water. When compared to controls, all ozone concentrations were effective in reducing bacteria.