M <sup>6</sup> A-mediated up-regulation of LncRNA LIFR-AS1 enhances the progression of pancreatic cancer via miRNA-150-5p/ VEGFA/Akt signaling
Jianqing Chen, Yuan‐Ping Tao, Yonggang Hong, Huifen Li, Zhiping Huang, Xuanfu Xu, Liang-Kai Hu
Abstract
A hyper-methylation on the 3' UTR of LIFR-AS1 to enhance its mRNA stability and LIFR-AS1 could directly interact with miR-150-5p, thereby indirectly up-regulating VEGFA expressions within cells. Through rescue experiments, we were able to confirm that the unfavorable impact of LIFR-AS1 knockdown on VEGFA /PI3K/Akt Signaling could be reversed via the inhibition of miR-150-5p expression. Together, these findings indicate that a noval m6A-LIFR-AS1 axis promotes PC progression at least in part via regulation of the miR-150-5p/VEGFA axis, indicating that this regulatory axis may be a viable clinical target for the treatment of PC.
Topics & Concepts
Gene knockdownLeukemia inhibitory factor receptorBiologyCancer researchmicroRNAProtein kinase BPI3K/AKT/mTOR pathwayCompeting endogenous RNACarcinogenesisVascular endothelial growth factor ACell biologyLong non-coding RNASignal transductionDownregulation and upregulationCancerCell cultureImmunologyVascular endothelial growth factorGeneticsVEGF receptorsLeukemia inhibitory factorGeneCytokineInterleukin 6RNA modifications and cancerCancer-related molecular mechanisms researchCancer-related gene regulation