Near-infrared rhodol dyes bearing salicylaldehyde moieties for ratiometric pH sensing in live cells during mitophagy and under hypoxia conditions
Sushil K. Dwivedi, Dilka Liyana Arachchige, Tara Vohs, Jiani Tang, Kyle Usimaki, Adenike Mary Olowolagba, Delaney Raine Fritz, Rudy L. Luck, Thomas Werner, Haiying Liu
Abstract
We describe a simple but efficient approach to make fluorescent probes A and B based on rhodol dyes incorporated with salicyaldehyde moiety for monitoring pH changes in mitochondria under oxidative stresses and hypoxia conditions, and for tracking mitophagy processes.
Topics & Concepts
MitophagySalicylaldehydeMoietyFluorescenceHypoxia (environmental)ChemistryInfraredOxidative phosphorylationMitochondrionOxygenPhotochemistryBiophysicsBiochemistryPolymer chemistryStereochemistryOrganic chemistryBiologyOpticsPhysicsAutophagySchiff baseApoptosisMolecular Sensors and Ion DetectionLuminescence and Fluorescent MaterialsSulfur Compounds in Biology