Purification and Characterization of the Isoprene Monooxygenase from <i>Rhodococcus</i> sp. Strain AD45
Leanne P. Sims, Colin W. J. Lockwood, Andrew T. Crombie, Justin M. Bradley, Nick E. Le Brun, J. Colin Murrell
Abstract
Isoprene is a highly abundant climate-active gas and a carbon source for some bacteria. Analyses of the genes encoding isoprene monooxygenase (IsoMO) indicate this enzyme is a soluble diiron center monooxygenase in the same family of oxygenases as soluble methane monooxygenase, alkene monooxygenase, and toluene monooxygenase.
Topics & Concepts
IsoprenePseudomonas putidaMonooxygenaseBiochemistryRhodococcusMethane monooxygenaseOxygenaseChemistryBacteriaEscherichia coliDioxygenaseBiologyStereochemistryEnzymeOrganic chemistryGeneCytochrome P450GeneticsPolymerCopolymerMicrobial bioremediation and biosurfactantsMetal-Catalyzed Oxygenation MechanismsRadioactive element chemistry and processing