<i>spa</i> Typing of Methicillin-Resistant Staphylococcus aureus Based on Whole-Genome Sequencing: the Impact of the Assembler
Sarah Mollerup, Peder Worning, Andreas Petersen, Mette Damkjær Bartels
Abstract
spa typing of methicillin-resistant Staphylococcus aureus (MRSA) is widely used by clinicians, infection control workers, and researchers both in local outbreak investigations and as an easy way to communicate and compare MRSA types between laboratories and countries. Traditionally, spa types are determined by Sanger sequencing, but in recent years a whole-genome sequencing (WGS)-based approach has become increasingly used.
Topics & Concepts
Staphylococcus aureusTypingMethicillin-resistant Staphylococcus aureusWhole genome sequencingGenomeBiologyGeneticsMicrobiologyComputational biologyGeneBacteriaAntimicrobial Resistance in StaphylococcusBacterial biofilms and quorum sensingBacterial Identification and Susceptibility Testing