Coumarin Exhibits Broad-Spectrum Antibiofilm and Antiquorum Sensing Activity against Gram-Negative Bacteria: <i>In Vitro</i> and <i>In Silico</i> Investigation
Faizan Abul Qais, Mohammad Shavez Khan, Iqbal Ahmad, Fohad Mabood Husain, Rais Ahmad Khan, Iftekhar Hassan, Syed Ali Shahzad, Walaa Alharbi
Abstract
studies where coumarin formed a stable complex with the tested proteins. The secondary structure of all proteins showed a negligible change in the presence of coumarin. Computational studies showed that the possible mechanisms of anti-QS activity were the inhibition of AHL synthesis, antagonization of QS-regulatory proteins, and blocking of the receptor proteins. The findings of this study clearly highlight the potency of coumarin against the virulence factors of Gram-negative bacterial pathogens that may be developed as an effective inhibitor of QS and biofilms.
Topics & Concepts
Quorum sensingCoumarinSwarming motilityPyocyaninBiofilmPseudomonas aeruginosaMicrobiologySerratia marcescensVirulencePyoverdineDocking (animal)Chromobacterium violaceumBacteriaBiologyMode of actionChemistryBiochemistryEscherichia coliMedicineGeneBotanyGeneticsNursingBacterial biofilms and quorum sensingAntibiotic Resistance in BacteriaAntimicrobial Resistance in Staphylococcus