Cell-free protein synthesis in hydrogel materials
Colette J. Whitfield, Alice M. Banks, Gema Durá, John Love, Jonathan E. Fieldsend, Sarah A. Goodchild, David A. Fulton, Thomas P. Howard
Abstract
We report a method for embedding cell-free protein synthesis reactions in macro-scale hydrogel materials without a free liquid phase. This paper focuses on methods of preparation for a variety of hydrogels and an investigation of the impact that the hydrogel material has on cell-free protein synthesis.
Topics & Concepts
Self-healing hydrogelsCovalent bondChemistryPolysaccharideNanotechnologyMaterials sciencePolymer chemistryBiochemistryOrganic chemistryGlycosylation and Glycoproteins ResearchSupramolecular Self-Assembly in MaterialsBacteriophages and microbial interactions