Circular <scp>RNA</scp> has_circ_0000527 participates in proliferation, invasion and migration of retinoblastoma cells via <scp>miR</scp>‐646/<scp>BCL</scp>‐2 axis
Nan‐nan Chen, Dai‐ling Chao, Xiu‐gui Li
Abstract
Retinoblastoma (RB) is one of the most common primary intraocular malignancies in children. Emerging researches have shown that circular RNAs (circRNAs) play critical roles in a variety of cancers. As a novel circRNA, the function of circ_0000527 in RB remains unknown. In this work, expression level of circ_0000527 and miR-646 in RB tissues and cell lines were detected by quantitative real-time polymerase chain reaction (qRT-PCR). RB cell lines (SO-Rb50 and WERI-Rb-1) were used as cell models in functional experiments. CCK-8 assay, TUNEL assay and transwell assay were employed to detect the biological influence of circ_0000527 and miR-646 on cancer cells in vitro. qRT-PCR, luciferase reporter assay, RIP assay and western blot were used for exploring the interactions among circ_0000527, miR-646 and BCL-2. It was demonstrated that expression level of circ_0000527 in RB samples was significantly up-regulated compared to normal tissues, while miR-646 was markedly down-regulated. Overexpression of circ_0000527 promoted the viability, migration and invasion of RB cells, while miR-646 transfection had the opposite effects. Circ_0000527 sponged miR-646 to regulate the expression of BCL-2. In conclusion, circ_0000527 could promote the development of RB by indirectly modulating BCL-2 via absorbing miR-646. SIGNIFICANCE OF THE STUDY: Expression level of circ_0000527 in RB samples was significantly up-regulated compared to normal tissues, while miR-646 was markedly down-regulated. Overexpression of circ_0000527 promoted the viability, migration and invasion of RB cells, while miR-646 transfection had the opposite effects. Circ_0000527 sponged miR-646 to regulate the expression of BCL-2.