Donor-specific phenotypic variation in hiPSC cardiomyocyte-derived exosomes impacts endothelial cell function
Amy Turner, Praful Aggarwal, Andrea Matter, Benjamin Olson, C. Charles Gu, Steven C. Hunt, Cora E. Lewis, Donna K. Arnett, Rachel Lorier, Ulrich Broeckel
Abstract
The intracellular RNA and the miRNA content in exosomes are significantly different in hiPSC-CMs derived from LVH-affected individuals compared with those from unaffected individuals. Treatment of endothelial cells with these exosomes functionally affects cellular phenotypes in a donor-specific manner. These findings provide novel insight into underlying mechanisms of hypertrophic cell signaling between different cell types. With a growing interest in stem cells and exosomes for cardiovascular therapeutic use, this also provides information important for regenerative medicine.
Topics & Concepts
MicrovesiclesCell biologyBiologyAngiogenesisInduced pluripotent stem cellCell typeIntracellularEndothelial stem cellExosomeCellmicroRNACancer researchGeneticsIn vitroEmbryonic stem cellGeneExtracellular vesicles in diseaseMicroRNA in disease regulationRenal and related cancers