Development of a highly efficient prime editor 2 system in plants
Juan Li, Like Chen, Jing Liang, Rongfang Xu, Yingli Jiang, Yizhen Li, Jian Ding, Min Li, Ruiying Qin, Pengcheng Wei
Abstract
Abstract Low efficiency has seriously restricted the application of prime editing (PE) systems in plants. In this study, we develop an enhanced plant prime editor 2 system, enpPE2, by stacking various optimization strategies, including updating the PE architecture to PEmax and expressing engineered pegRNA with a structured motif under the control of a composite promoter. In T 0 rice plants, enpPE2 exhibits editing frequencies of 64.58% to 77.08%, which are much higher than the frequencies with unmodified pPE2. Our results indicate that the enpPE2 system provides a robust and powerful tool for the precise modification of plant genomes.