Circular RNA 100146 Promotes Colorectal Cancer Progression by the MicroRNA 149/HMGA2 Axis
Kunpeng Liu, Yuhua Mou, Xiufang Shi, Tingkun Liu, Zhanfeng Chen, Xingmei Zuo
Abstract
Also, miR-149 was negatively regulated by circRNA 100146 and was targeted to HMGA2 and mediated its expression. Moreover, miR-149 interference abrogated the activities of silenced circRNA 100146 in proliferation, apoptosis, migration, and invasion. Furthermore, HMGA2 overexpression abated the effects described above caused by circRNA 100146 silencing, while the mutations on miR-149 binding sites in the 3' untranslated region (3'-UTR) of HMGA2 led to its loss of this ability. circRNA 100146 knockdown repressed proliferation, enhanced apoptosis, and hindered migration and invasion in SW620 and SW480 cells through targeting the miR-149/HMGA2 axis.
Topics & Concepts
Gene knockdownmicroRNABiologyGene silencingCircular RNAHMGA2Cell growthCancer researchApoptosisMolecular biologyRNA interferenceRNAGeneGeneticsCircular RNAs in diseasesMicroRNA in disease regulationCancer-related molecular mechanisms research