Salivary annexin <scp>A1</scp>: A candidate biomarker for periodontitis
Renato Côrrea Viana Casarin, Cristiane Ribeiro Salmon, Camila S. Stolf, Hélvis E. S. Paz, Thiago Perez Rangel, Romênia R. Domingues, Bianca Alves Pauletti, Adriana Franco Paes Leme, Cássia Fernandes Araújo, Mauro Pedrine Santamaría, Karina S. Ruiz, Mabelle Freitas Monteiro
Abstract
AIM: To compare the salivary proteomic profile of periodontitis-affected (PA) parents and their offspring to periodontally healthy (PH) dyads in the pursuit of possible biomarkers for early diagnosis of this disease. MATERIALS AND METHODS: Unstimulated saliva samples collected from 17 pairs of PA or PH individuals and their children were submitted to mass spectrometric analyses followed by proteomic analyses. Primary PA fibroblasts were triggered towards having an inflammatory response, and an immunoenzymatic assay of its supernatant was performed to validate the obtained data. RESULTS: ANXA1, KRT4, GSTP1, HPX, A2M and KRT13 were lower in PA parents and their children, and IGHG1, CSTB, KRT9, SMR3B, IGHG4 and SERPINA1 were higher. ANXA1 presented the highest fold change, 7.1 times less produced in children of PA parents, and was selected as a potential biomarker for periodontitis. The in vitro assay also showed lower ANXA1 production by cells of PA patients. CONCLUSION: Before any clinical sign of periodontal loss, descendants of PA patients have an altered proteomic profile compared to PH individuals, presenting a lower abundance of ANXA1. This protein is suggested as a potential biomarker for periodontitis.