Rapid and facile detection of HBV with CRISPR/Cas13a
Ronghua Ding, Yue Shen, Mingzhu Yuan, Xue Zheng, Shuaiyin Chen, Guangcai Duan
Abstract
A rapid point-of-care assay based on CRISPR-Cas13a for HBV was built with a LOD of 1 copy per μL was achieved in 50 min. The lateral flow test strip method can achieve naked-eye results, and the fluorescence readout can achieve real-time detection.
Topics & Concepts
ChemistryCRISPRPoint-of-care testingNaked eyeFluorescencePoint of careDetection limitChromatographyGenePhysicsOpticsNursingImmunologyBiologyMedicineBiochemistryCRISPR and Genetic EngineeringMosquito-borne diseases and controlVibrio bacteria research studies