Rapid detection of <i>Vibrio parahaemolyticus</i> using magnetic nanobead-based immunoseparation and quantum dot-based immunofluorescence
Yue Zhai, Xiangjun Meng, Li Li, Yushen Liu, Kun Xu, Chao Zhao, Juan Wang, Xiuling Song, Juan Li, Minghua Jin
Abstract
IMB probe and QD probe were used as separation and fluorescent label to measure the content of V. parahaemolyticus . The detection method with a detection limit of 10 2 cfu mL −1 has been shown to work satisfactorily in real food samples.
Topics & Concepts
Vibrio parahaemolyticusDetection limitFluorescenceQuantum dotImmunofluorescenceMeasure (data warehouse)Limit (mathematics)ChemistryChromatographyMicrobiologyPhysicsOptoelectronicsBiologyOpticsBacteriaMathematicsComputer scienceImmunologyAntibodyMathematical analysisDatabaseGeneticsVibrio bacteria research studiesSalmonella and Campylobacter epidemiologyAquaculture disease management and microbiota