PEARL-seq: A Photoaffinity Platform for the Analysis of Small Molecule-RNA Interactions
H. G. MUKHERJEE, J. Craig Blain, Lee E. Vandivier, Donovan N. Chin, Jessica E. Friedman, Fei Liu, Ashley Maillet, Chao Fang, Jenifer B. Kaplan, Jinxing Li, David M. Chenoweth, Allan Beck Christensen, L. Petersen, Nils Jakob Vest Hansen, Luis Barrera, Neil Kubica, G. Kumaravel, Jennifer C. Petter
Abstract
uencing (PEARL-seq), enables the rapid identification of small molecule binding locations within their RNA targets and can provide information on ligand selectivity across multiple different RNAs. These data, when supplemented with small molecule SAR data and RNA probing data enable the construction of a computational model of the RNA-ligand structure, thereby enabling the rational design of novel RNA-targeted ligands.
Topics & Concepts
RNASmall moleculeRational designComputational biologyPhotoaffinity labelingSmall RNALigand (biochemistry)BiologyChemistryBinding siteBiochemistryGeneGeneticsReceptorRNA and protein synthesis mechanismsRNA modifications and cancerRNA Research and Splicing