Characterization of chemical constituents in Shuanghuanglian oral dosage forms by ultra‐high performance liquid chromatography coupled with time‐of‐flight mass spectrometry
Ruiyun Li, Yanru Zhu, Mingyan Ma, Min Lei, Yingchun Zhao, Yingchun Zhao, Ting Liu, Miao Yu, Yunli Zhao, Yunli Zhao, Zhiguo Yu
Abstract
Shuanghuanglian is a common traditional Chinese medicine prescription. It is an herbal formula composed of Lonicerae Japonicae Flos, Scutellariae Radix, and Forsythiae Fructus. A comprehensive understanding of Shuanghuanglian oral dosage forms components was obtained using a method based on ultra-high performance liquid chromatography coupled with time-of-flight mass spectrometry for the separation and characterization of Shuanghuanglian oral liquids, granules, soft capsules, and effervescent tablets. A total of 358 components were chemically defined or tentatively identified, including flavonoids, caffeic acid derivatives, lignans, coumarins, iridoids, triterpenes, and anthraquinones. The results will provide a basis for the general study of Shuanghuanglian and be meaningful for the composition identification of traditional Chinese medicine prescriptions.