ISOLATION AND IDENTIFICATION OF PROTEASEPRODUCING BACILLUS STRAIN FROM COLD CLIMATE SOIL AND OPTIMIZATION OF ITS PRODUCTION BY APPLYING DIFFERENT FERMENTATION CONDITIONS
Nazia Khatoon, Nazif Ullah, Abid Sarwar, Shafiq Ur Rahman, Ahmed Abdul Haleem Khan, Tariq Aziz, M. ALHARBI, Abdulrahman Alshammari
Abstract
Proteases have received a lot of attention in recent years thanks to their use in a variety of industries, including the food, feed, leather, textile, and pharmaceutical sectors.The soil sample from a cold climate was used in the current experimental study of protease production.In order to identify the protease-producing bacterial species, morphological, physiological, and biochemical analyses were carried out, and the isolated bacterial strain's ability to produce proteases on skim milk agar was assessed.As a result of casein hydrolysis, a zone formed surrounding the bacterial colony suggested a protease-positive strain.To increase the production of protease by isolated Bacillus species, various sources of nitrogen and carbon were examined.Yeast extract (309.32IU/ml) was the nitrogen source that supported growth the best among the other sources studied, and fructose (269.4IU/ml) was the carbon source that produced the most protease activity.The fermentation time of 72 h (393.7033IU/ml) was anticipated to be the best setting for protease synthesis.When 2% (w/v) soybean meal was employed, protease activity was measured at 494.92 IU/ml.The influence of pH was investigated, and it was discovered that 7.5 was the best and optimal pH for Bacillus species to produce 511 IU/ml of protease.When the temperature range was discovered to be 40°C, the highest protease activity of 542.93 IU/ml was recorded.