Environmental Hot Spots and Resistance-Associated Application Practices for Azole-Resistant <i>Aspergillus fumigatus</i>, Denmark, 2020–2023
Maiken Cavling Arendrup, Rasmus Krøger Hare, Karin Meinike Jørgensen, Ulla E. Bollmann, Tina B. Bech, Cecilie Cetti Hansen, Thies Marten Heick, Lise Nistrup Jørgensen
Abstract
/Y121F/T289A-related mechanisms), constituting 4.2% of 4,538 A. fumigatus isolates. The highest proportions were in flower- and compost-related samples but were not correlated with azole-fungicide application concentrations. Genotyping showed clustering of tandem repeat-related ARAf and overlaps with clinical isolates in Denmark. A. fumigatus fungi grew poorly in the field experiment with no postapplication change in ARAf proportions. However, in microcosmos experiments, a sustained complete (tebuconazole) or partial (prothioconazole) inhibition against wild-type A. fumigatus but not ARAf indicated that, under some conditions, azole fungicides may favor growth of ARAf in soil.