CTCF-binding element regulates ESC differentiation via orchestrating long-range chromatin interaction between enhancers and HoxA
Guangsong Su, Wenbin Wang, Jun Chen, Man Liu, Jian Zheng, Dianhao Guo, Jinfang Bi, Zhongfang Zhao, Jiandang Shi, Lei Zhang, Wange Lu
Abstract
organizes chromatin interactions between its adjacent enhancers and HoxA. Furthermore, deletion of those adjacent enhancers synergistically inhibited HoxA activation, suggesting that these enhancers serve as an EEIC required for RA-induced HoxA activation. Collectively, these results provide new insight into RA-induced HoxA expression during early ESC differentiation, also highlight precise regulatory roles of the CTCF-binding element in orchestrating high-order chromatin structure.
Topics & Concepts
EnhancerCTCFChromatinBiologyCell biologyHomeoboxCellular differentiationGeneticsRegulation of gene expressionTranscription factorGeneGenomics and Chromatin DynamicsRNA Research and SplicingCRISPR and Genetic Engineering