Arabidopsis Flippases Cooperate with ARF GTPase Exchange Factors to Regulate the Trafficking and Polarity of PIN Auxin Transporters
Xixi Zhang, Maciek Adamowski, Petra Marhavá, Shutang Tan, Yuzhou Zhang, Lesia Rodríguez, Marta Zwiewka, Vendula Pukyšová, Adrià Sans Sánchez, Vivek K. Raxwal, Christian S. Hardtke, Tomasz Nodzyński, Jiřı́ Friml
Abstract
T-DNA mutants show defects in many auxin-regulated processes, asymmetric auxin distribution, and PIN trafficking. Analysis of quintuple and sextuple mutants confirmed the crucial roles of ALA proteins in regulating plant development as well as PIN trafficking and polarity. Genetic and physical interaction studies revealed that ALA3 functions together with the ADP ribosylation factor GTPase exchange factors GNOM and BIG3 in regulating PIN polarity, trafficking, and auxin-mediated development.
Topics & Concepts
BiologyArabidopsisCell biologyAuxinCell polarityPolarity (international relations)MutantGTPaseArabidopsis thalianaPolar auxin transportGenetic screenMutationGeneticsCellGenePlant Molecular Biology ResearchPlant Reproductive BiologyPhotosynthetic Processes and Mechanisms