Virulence and Cross-Protection Conferred by an Attenuated Genotype I-Based Chimeric Japanese Encephalitis Virus Strain Harboring the E Protein of Genotype V in Mice
Qiqi Xia, Yan Zhang, Yang Yang, Xiaochun Ma, Zhixin Guan, Junjie Zhang, Zongjie Li, Ke Liu, Beibei Li, Donghua Shao, Yafeng Qiu, Jianchao Wei, Zhiyong Ma
Abstract
The GV JEV showed an increase in epidemic areas, which exhibited higher pathogenicity in mice than the prevalent GI and GIII strains. We replaced a recombinant chimeric GI-GV JEV (JEV-GI/V) strain to determine the role of the structural proteins in virulence and cross-protection. It was found that the essential role of the structural proteins is to determinethe virulence of the GV strain. It is also suggested that there is reduced cross-protection of JEV vaccines among different genotypes, which provides basic data for subsequent JEV prevention, control, and new vaccine development.
Topics & Concepts
VirulenceVirologyJapanese encephalitisBiologyVirusGenotypeTiterRecombinant DNAHeterologousNeutralizing antibodyEncephalitisGeneGeneticsMosquito-borne diseases and controlViral Infections and VectorsVector-borne infectious diseases