A rapid and sensitive method for chiroptical sensing of α-amino acids <i>via</i> click-like labeling with <i>o</i> -phthalaldehyde and <i>p</i> -toluenethiol
Bo Li, Jie Zhang, Li Li, Gong Chen
Abstract
group of analytes into an isoindole. The covalent labeling generates sensitive UV and CD readouts, both of which show an excellent linear relationship with the concentration of analytes. The high reactivity and the novel optical reporting mechanism allow fast and accurate measurement without background interference. The sensing assay works well for a remarkably broad range of analyte concentrations, with an unprecedented lower limit of 10 micromolar concentration.
Topics & Concepts
IsoindoleAnalyteChemistryO-PhthalaldehydeReagentCombinatorial chemistryCovalent bondLinear rangeDetection limitChromatographyDerivatizationStereochemistryHigh-performance liquid chromatographyOrganic chemistryMolecular Sensors and Ion DetectionLuminescence and Fluorescent MaterialsChemical Synthesis and Analysis