lncRNA <i>PVT1</i> Promotes Metastasis of Non-Small Cell Lung Cancer Through EZH2-Mediated Activation of Hippo/NOTCH1 Signaling Pathways.
Shaopeng Zeng, Xie Jian-hong, Qun-Ying Zeng, Shuang Dai, Yun Wang, Xuemei Wan, Ji C Hun Liu
Abstract
OBJECTIVE: in regulating the tumorigenesis and development of NSCLC. MATERIALS AND METHODS: with enhancer of zeste homolog 2 (EZH2) was verified by RNA pull-down, and their binding to the downstream targets was detected by Chromatin Immunoprecipitation (ChIP) assays. RESULTS: transcription and its target YAP1 upregulation, and finally NOTCH signaling pathway was activated, which promoted EMT and invasion and metastasis. CONCLUSION: promotes NSCLC metastasis through EZH2-mediated activation of Hippo/NOTCH1 signaling pathways. This study provides a new opportunity to advance our understanding in the potential mechanism of NSCLC development.
Topics & Concepts
BiologyYAP1Hippo signaling pathwayPVT1Cancer researchCarcinogenesisMatrigelChromatin immunoprecipitationSignal transductionNotch signaling pathwayGene knockdownTranscription factorCell biologyDownregulation and upregulationCell cultureLong non-coding RNAGene expressionCancerAngiogenesisPromoterGeneticsGeneCancer-related molecular mechanisms researchRNA Research and SplicingHippo pathway signaling and YAP/TAZ