Creating rat hepatocyte organoid as an <i>in vitro</i> model for drug testing
Yuting He, Xinglong Zhu, Shengfu Li, Bing‐Qi Zhang, Yi Li, Qiong Wu, Yunlin Zhang, Yanyan Zhou, Li Li, Yana Qi, Ji Bao, Hong Bu
Abstract
BACKGROUND: the MSC-based traction force triggered by extracellular matrix (ECM) proteins. In this study, primary hepatocytes were co-cultured with MSCs on a liver-derived ECM to generate liver organoids within a short duration. AIM: To create hepatocyte organoids by co-culturing primary hepatocytes with MSCs on a porcine liver extracellular matrix (PLECM) gel. METHODS: gene markers, and urea cycle genes. Culture medium was collected to detect albumin (ALB) and urea production on days 2, 4, 6, 8, 14, and 20. RESULTS: . CONCLUSION: Our new method of creating primary hepatocyte organoids by co-culturing hepatocytes with MSCs on liver-derived ECM hydrogels could be used to develop models for liver disease and for drug screening.