2′3′-cGAMP triggers a STING- and NF-κB–dependent broad antiviral response in <i>Drosophila</i>
Hua Cai, Andreas Holleufer, Bine Simonsen, Juliette Schneider, Aurélie Lemoine, Hans Henrik Gad, Jingxian Huang, Jieqing Huang, Di Chen, Tao Peng, João T. Marques, Rune Hartmann, Nelson Martins, Jean‐Luc Imler
Abstract
, genes that encode key components of the autophagy and small interfering RNA pathways, respectively. By contrast, this protection was abrogated in flies with mutations in the gene encoding the NF-κB transcription factor Relish. Transcriptomic analysis of 2'3'-cGAMP-injected flies revealed a complex response pattern in which genes were rapidly induced, induced after a delay, or induced in a sustained manner. Our results reveal that dSTING regulates an NF-κB-dependent antiviral program that predates the emergence of interferons in vertebrates.