<i>In Vitro</i> Modelling of Oral Microbial Invasion in the Human Colon
Lucie Etienne‐Mesmin, Victoria Meslier, Ophélie Uriot, E Fournier, Charlotte Deschamps, Sylvain Denis, Aymeric David, Sarah Jégou, Christian Morabito, Benoît Quinquis, Florence Thirion, Florian Plaza Oñate, Emmanuelle Le Chatelier, S. Dusko Ehrlich, Stéphanie Blanquet‐Diot, Mathieu Almeida
Abstract
model simulating both the physicochemical and microbial (lumen- and mucus-associated microbes) parameters of the human colon (M-ARCOL), a salivary enrichment protocol, and whole-metagenome shotgun sequencing. Our study revealed the importance of integrating the mucus compartment, which retained higher microbial richness during fermentation, showed the preference of oral microbial invaders for the mucosal resources, and indicated potential oral-to-intestinal mucosal competitions. It also underlined promising opportunities to further understand mechanisms of oral invasion into the human gut microbiome, define microbe-microbe and mucus-microbe interactions in a compartmentalized fashion, and help to better characterize the potential of oral microbial invasion and their persistence in the gut.