Automatic and multi-channel detection of bacteria on a slidable centrifugal disc based on FTA card nucleic acid extraction and recombinase aided amplification
Siyuan Wang, Gaozhe Cai, Hong Duan, Qi Chen, Jianhan Lin
Abstract
This study developed a slidable centrifugal disc for automatic and multi-channel detection of Salmonella using FTA card for DNA extraction and RAA for DNA detection and verified it could detect 10 CFU mL −1 in chicken meat supernatant in 1 h.
Topics & Concepts
Nucleic acidExtraction (chemistry)Channel (broadcasting)BacteriaChemistryChromatographyComputer scienceBiologyBiochemistryGeneticsComputer networkBiosensors and Analytical DetectionMicrofluidic and Capillary Electrophoresis ApplicationsAdvanced biosensing and bioanalysis techniques