Rapid Detection of DNA and RNA Shrimp Viruses Using CRISPR-Based Diagnostics
Samuel R. Major, Matthew J. Harke, Roberto Cruz‐Flores, Arun K. Dhar, Andrea G. Bodnar, Shelly A. Wanamaker
Abstract
The Pacific white shrimp (Penaeus vannamei) is one of the most valuable aquaculture species in the world but has suffered major economic losses from outbreaks of White Spot Syndrome Virus and Taura Syndrome Virus. Rapid detection of these viruses can improve aquaculture practices by enabling more timely action to be taken to combat disease outbreaks. Highly sensitive, specific, and robust CRISPR-based diagnostic assays such as those developed here have the potential to revolutionize disease management in agriculture and aquaculture helping to promote global food security.
Topics & Concepts
ShrimpOutbreakWhite spot syndromeCRISPRBiologyAquaculturePenaeusVirusVirologyFisheryFish <Actinopterygii>GeneGeneticsCRISPR and Genetic EngineeringInvertebrate Immune Response MechanismsAquaculture disease management and microbiota