Laser-Induced Shockwave (LIS) to Study Neuronal Ca2+ Responses
Veronica Gomez Godinez, Vikash Morar, Christopher Carmona, Yingli Gu, Kijung Sung, Linda Shi, Chengbiao Wu, Daryl Preece, Michael W. Berns
Abstract
Laser-induced shockwaves (LIS) can be utilized as a method to subject cells to conditions similar to those occurring during a blast-induced traumatic brain injury. The pairing of LIS with genetically encoded biosensors allows researchers to monitor the immediate molecular events resulting from such an injury. In this study, we utilized the genetically encoded Ca 2+ FRET biosensor D3CPV to study the immediate Ca 2+ response to laser-induced shockwave in cortical neurons and Schwann cells. Our results show that both cell types exhibit a transient Ca 2+ increase irrespective of extracellular Ca 2+ conditions. LIS allows for the simultaneous monitoring of the effects of shear stress on cells, as well as nearby cell damage and death.