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Oral tryptophan activates duodenal aryl hydrocarbon receptor in healthy subjects: a crossover randomized controlled trial

Gaston Rueda, Natalia Causada Calo, Rajka Borojevic, Andrea Nardelli, María Inés Pinto-Sánchez, Marco Constante, Josie Libertucci, Vidhyalakshmi Mohan, Philippe Langella, Linda M. P. Loonen, Jerry M. Wells, Stephen M. Collins, Harry Sokol, Elena F. Verdú, Přemysl Berčík

2024American Journal of Physiology-Gastrointestinal and Liver Physiology23 citationsDOIOpen Access PDF

Abstract

We demonstrate that in healthy subjects, orally administered tryptophan activates microbial indole and host kynurenine pathways in the small intestine, the primary metabolic site for dietary components, and the richest source of immune cells along the gut. This study provides novel insights in how to optimally activate immunomodulatory AhR pathways and indole metabolism in the small intestine, serving as basis for future therapeutic trials using l-tryptophan supplementation in chronic inflammatory conditions affecting the small intestine.

Topics & Concepts

Aryl hydrocarbon receptorTryptophanKynurenineCrossover studyKynurenine pathwayPlaceboInternal medicineMetaboliteEndocrinologyPharmacologySerotoninChemistryBiologyMedicineReceptorBiochemistryAmino acidPathologyTranscription factorGeneAlternative medicineTryptophan and brain disordersGut microbiota and healthDiet and metabolism studies