Genome sequence of Leishmania mexicana MNYC/BZ/62/M379 expressing Cas9 and T7 RNA polymerase
Tom Beneke, Ulrich Dobramysl, Carolina Moura Costa Catta‐Preta, Jeremy C. Mottram, Eva Gluenz, Richard John Wheeler
Abstract
MNYC/BZ/62/M379 modified to express Cas9 and T7 RNA-polymerase, revealing high similarity to the reference genome (MHOM/GT2001/U1103). Through RNAseq-based annotation of coding sequences and untranslated regions, we provide primer sequences for construct and sgRNA template generation for CRISPR-assisted gene deletion and endogenous tagging.
Topics & Concepts
BiologyGenomeGeneticsGeneGuide RNAT7 RNA polymeraseComputational biologyCas9BacteriophageEscherichia coliCRISPR and Genetic Engineering