Modulation by Co (II) Ion of Optical Activities of <i>L</i>/<i>D</i>‐glutathione (GSH)‐modified Chiral Copper Nanoclusters for Sensitive Adenosine Triphosphate Detection
Qi Ding, Weimin Yang, Xinhe Xing, Hengwei Lin, Chuanlai Xu, Liguang Xu, Si Li
Abstract
Abstract Chiral nanoscale enantiomers exhibit different biological effects in living systems. However, their chirality effect on the detection sensitivity for chiral biological targets still needs to be explored. Here, we discovered that Co 2+ can modulate the luminescence performance of L / D ‐glutathione (GSH)‐modified copper nanoclusters ( L / D −Cu NCs) and induce strong chiroptical activities as the asymmetric factor was enhanced 223‐fold with their distribution regulating from the ultraviolet to visible region. One Co 2+ coordinated with two GSH molecules that modified on the surface of Cu NCs in the way of CoN 2 O 2 . On this basis, dual‐modal chiral and luminescent signals of Co 2+ coordinated L / D −Cu NCs ( L / D −Co−Cu NCs) were used to detect the chiral adenosine triphosphate (ATP) based on the competitive interaction between surficial GSH and ATP molecules with Co 2+ . The limits of detection of ATP obtained with fluorescence and circular dichroism intensity were 9.15 μM and 15.75 nM for L −Co−Cu NCs, and 5.35 μM and 4.69 nM for D −Co−Cu NCs. This demonstrated that selecting suitable chiral configurations of nanoprobes effectively enhances detection sensitivity. This study presents not only a novel method to modulate and enhance the chiroptical activity of nanomaterials but also a unique perspective of chirality effects on the detection performances for bio‐targets.