Characterization of polyvinylpyrrolidone (PVP) with technetium-99m and its accumulation in mice
A. Massoud, Safaa B. Challan, Nabila A. Maziad
Abstract
Polyvinylpyrrolidone (PVP) is a biopolymer compound that has been widely used in pharmaceutical and medical applications, especially as a tablet binder and solubilizing agents for injections. In this work, PVP was characterized by FTIR to confirm its structure and Dynamic light scattering (DLS) to determine its mean diameter for studying concentration ranges. PVP was radio-labeled with Technetium-99m using SnCl2.2H2O as a reducing agent with an efficiency of (97 ± 0.15) % at the best conditions. The stability 99mTc-PVP complex was measured in serum and saline media in vitro for 24 hours and it was found that the complex had good stability properties for 24 hours. The biological distribution of the 99mTc-PVP complex was studied in normal mice using oral and intravenous injection to discover its accumulation in different mice organs. The results indicated that the rate of the 99mTc-PVP complex in the blood decreased rapidly during the first three hours after injection, and also, it was mainly excreted by the kidneys with negligible uptake in the brain. The results also confirmed that the 99mTc-PVP complex was accumulated with high percentages in the urinary bladder during the 3 hours post-injection.