Insights into the Unique Lung Microbiota Profile of Pulmonary Tuberculosis Patients Using Metagenomic Next-Generation Sequencing
Guohui Xiao, Zhao Cai, Qinglong Guo, Taosheng Ye, Yimin Tang, Peikun Guan, Juanjuan Zhang, Min Ou, Xiangdong Fu, Lili Ren, Minfei Yu, Zhaoqin Wang, Lei Liu, Liang Yang, Guoliang Zhang
Abstract
Characterization of the lung microbiota could lead to a better understanding of the pathogenesis of pulmonary TB. Here, we applied the metagenomic shotgun sequencing instead of 16S rRNA sequencing method to characterize the lung microbiota using the BALF samples instead of sputum. We found that alterations in the lung microbiota are associated with TB infection and that anti-TB treatment significantly affects the alpha and beta diversity of the lung microbiota in pulmonary TB patients. These findings could help us better understand TB pathogenesis.
Topics & Concepts
MetagenomicsSputumShotgun sequencingBiologyDNA sequencing16S ribosomal RNAComputational biologyPulmonary tuberculosisDeep sequencingLungMicrobiologyTuberculosisGeneticsMedicineBacteriaGenePathologyGenomeInternal medicineMycobacterium research and diagnosisGut microbiota and healthTuberculosis Research and Epidemiology