Enzymatic activity of individual bioelectrocatalytic viral nanoparticles: dependence of catalysis on the viral scaffold and its length
Telmo O. Paiva, Angela Schneider, Laure Bataille, Arnaud Chovin, Agnès Anne, Thierry Michon, Christina Wege, Christophe Demaille
Abstract
(previous work), reveals two distinct kinetic effects of scaffolding: An enhancement of catalysis that does not depend on the virus type and a modulation of substrate inhibition that depends on the virus type. AFM-SECM detection of the enzymatic activity of a few tens of PQQ-GDH molecules, decorating a 40 nm-long viral domain, is also demonstrated, a record in terms of the lowest number of enzyme molecules interrogated by an electrochemical imaging technique.
Topics & Concepts
ChemistryCatalysisBiophysicsScanning electrochemical microscopySubstrate (aquarium)EnzymeEnzyme catalysisElectrochemistryNanotechnologyCombinatorial chemistryMaterials scienceBiochemistryElectrodeBiologyPhysical chemistryEcologyAdvanced biosensing and bioanalysis techniquesBacteriophages and microbial interactionsBiosensors and Analytical Detection