Expression Profile and Localization of SARS-CoV-2 Nonstructural Replicase Proteins in Infected Cells
Fang‐Shu Shi, Yu Yin, Yali Li, Lilan Cui, Zhuangzhuang Zhao, Mi Wang, Bin Wang, Rong Zhang, Yao‐Wei Huang
Abstract
The intracellular localization of SARS-CoV-2 replicase nonstructural proteins (nsp) during infection has not been fully elucidated. In this study, we systematically analyzed the expression and subcellular localization of 11 distinct viral nsp and dsRNA over time in SARS-CoV-2-infected cells by using individual antibody against these replicase proteins. The data indicated that nsp gene expression is highly regulated in space and time, which could be useful to understand the function of viral replicases and future development of diagnostics and potential antiviral strategies against SARS-CoV-2.
Topics & Concepts
BiologyVirologyViral replicationRNA-dependent RNA polymeraseWestern blotCoronavirusSubcellular localizationRNA silencingColocalizationImmunoprecipitationImmunofluorescenceVirusRNACell biologyAntibodyGeneCoronavirus disease 2019 (COVID-19)RNA interferenceGeneticsPathologyDiseaseMedicineInfectious disease (medical specialty)SARS-CoV-2 and COVID-19 ResearchAnimal Virus Infections StudiesViral gastroenteritis research and epidemiology