Developmental validation of the ForenSeq MainstAY kit, MiSeq FGx sequencing system and ForenSeq Universal Analysis Software

Abstract

For human identification purposes, forensic genetics has primarily relied upon a core set of autosomal (and to a lesser extent Y chromosome) short tandem repeat (STR) markers that are enriched by amplification using the polymerase chain reaction (PCR) that are subsequently separated and detected using capillary electrophoresis (CE). While STR typing conducted in this manner is well-developed and robust, advances in molecular biology that have occurred over the last 15 years, in particular massively parallel sequencing (MPS) [1Margulies M. Egholm M. Altman W.E. Attiya S. Bader J.S. Bemben L.A. et al.Genome sequencing in microfabricated high-density picolitre reactors.Nature. 2005; 437 (Epub 20050731): 376-380https://doi.org/10.1038/nature03959Crossref PubMed Scopus (6008) Google Scholar, 2Shendure J. Ji H. Next-generation D.N.A. Sequencing.Nat. Biotechnol. 2008; 26: 1135-1145https://doi.org/10.1038/nbt1486Crossref PubMed Scopus (3112) Google Scholar, 3Metzker M.L. 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