Contemporary Considerations for Establishing Reference Methods for Antibacterial Susceptibility Testing
Romney M. Humphries, Linda Miller, Barbara Zimmer, Erika Matuschek, Janet A. Hindler
Abstract
milieu of bacterial infections, requires multiple days to perform, and is associated with subtle, difficult to control variability. In addition, new reference methods will soon be needed for novel agents whose activity cannot be evaluated by BMD (e.g., those that target virulence). Any new reference methods must be standardized, correlated with clinical efficacy and be recognized internationally by researchers, industry, and regulators. Herein, we describe current reference methods for in vitro assessment of antibacterial activity and highlight key considerations for the generation of novel reference methods.
Topics & Concepts
Broth microdilutionIn vivoAntibacterial agentIn vitroMedicineBiologyPharmacologyMicrobiologyAntibioticsMinimum inhibitory concentrationBiotechnologyBiochemistryBacterial Identification and Susceptibility TestingAntibiotic Resistance in BacteriaAntibiotics Pharmacokinetics and Efficacy