Degradation of glucosinolates in rapeseed meal by Lactobacillus delbrueckii and Bacillus subtilis
Zhenyu Zhang, Min Wen, Yaqi Chang
Abstract
Lactobacillus delbrueckii and Bacillus subtilis were employed as a new combination of strains to treat rapeseed meal by solid-state fermentation, aiming to efficiently degrade the glucosinolates, which are the main toxin in the meal. Single-factor tests and Response surface methodology (RSM) were used to optimize the fermentation parameters. Under the optimum fermentation parameters of 15% total injection volume of the mixture of Lactobacillus delbrueckii and Bacillus subtilis with a ratio of 2:1, bran content of 16%, feed to water ratio of 1:1.5, fermentation temperature of 36 °C and fermentation time of 72 h, the content of glucosinolates in rapeseed meal was decreased from 64.558 μmol/g to 3.473 μmol/g, reaching a high degradation rate (94.62%). The high detoxification rate by a consortium of Lactobacillus delbrueckii and Bacillus subtilis provides a bright application prospect in feed utilization of rapeseed meal.