Direct observation of humic acid-promoted hydrolysis of phytate through stabilizing a conserved catalytic domain in phytase
Xinfei Ge, Wenjun Zhang, Christine V. Putnis, Lijun Wang
Abstract
HA promotes enzymatic hydrolysis of phytate as shown by the increase in nucleation of P i -bearing particles, which is achieved by conformation change to stabilize a catalytic domain resulting from noncovalent phytase–HA interaction.
Topics & Concepts
PhytaseHydrolysisCatalysisChemistryNucleationEnzymeDomain (mathematical analysis)Humic acidCatalytic efficiencyBiochemistryOrganic chemistryFertilizerMathematical analysisMathematicsPhytase and its ApplicationsFolate and B Vitamins ResearchIron Metabolism and Disorders