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Computed structures of core eukaryotic protein complexes

Ian R. Humphreys, Jimin Pei, Minkyung Baek, Aditya Krishnakumar, Ivan Anishchenko, Sergey Ovchinnikov, Jing Zhang, Travis J. Ness, Sudeep Banjade, Saket R. Bagde, Viktoriya G. Stancheva, Xiaohan Li, Kaixian Liu, Zhi Zheng, Daniel J. Barrero, Upasana Roy, Jochen Kuper, I.S. Fernandez, Barnabás Szakál, Dana Branzei, Josep Rizo, Caroline Kisker, Eric C. Greene, Sue Biggins, Scott Keeney, Elizabeth A. Miller, J. Christopher Fromme, Tamara L. Hendrickson, Qian Cong, David Baker

2021Science575 citationsDOIOpen Access PDF

Abstract

Deep learning for protein interactions The use of deep learning has revolutionized the field of protein modeling. Humphreys et al . combined this approach with proteome-wide, coevolution-guided protein interaction identification to conduct a large-scale screen of protein-protein interactions in yeast (see the Perspective by Pereira and Schwede). The authors generated predicted interactions and accurate structures for complexes spanning key biological processes in Saccharomyces cerevisiae . The complexes include larger protein assemblies such as trimers, tetramers, and pentamers and provide insights into biological function. —VV

Topics & Concepts

Computational biologyProteomeSaccharomyces cerevisiaeEukaryotic cellYeastBiologyCoevolutionFunction (biology)Protein structureGeneticsEvolutionary biologyBiochemistryGeneFungal and yeast genetics researchRNA and protein synthesis mechanismsBioinformatics and Genomic Networks