The <i>Arabidopsis</i> epigenetic regulator ICU11 as an accessory protein of Polycomb Repressive Complex 2
Rebecca Bloomer, Claire E. Hutchison, Isabel Bäurle, James W. Walker, Xiaofeng Fang, Pumi Perera, Christos N. Velanis, Serin Gümüs, Christos Spanos, Juri Rappsilber, Xiaoqi Feng, Justin Goodrich, Caroline Dean
Abstract
Significance Epigenetic regulation of gene expression is associated with switching between chromatin states characterized by distinct histone modifications. Polycomb/Trithorax regulation involves the mutually exclusive H3K27me3/H3K36me3 modifications, but how these states are faithfully inherited through DNA replication, yet can switch from one to another, is still poorly understood. One mechanism that would aid switching is the association of histone methyltransferases with factors demethylating the opposing histone modification. Here, we show that an Arabidopsis 2-oxoglutarate–dependent dioxygenase, an activity associated with histone demethylation in other organisms, physically associates with the Polycomb Repressive Complex 2. We propose that physical association of histone methylation/demethylation activities will be generally important to coordinate switching between chromatin states.