Integrated analysis of m6A mRNA methylation in rats with monocrotaline-induced pulmonary arterial hypertension
Yunhong Zeng, Ting Huang, Wanyun Zuo, Dan Wang, Yonghui Xie, Xun Wang, Zhenghui Xiao, Zhi Chen, Qiming Liu, Na Liu, Yunbin Xiao
Abstract
BACKGROUND: A-related enzymes in pulmonary arterial hypertension (PAH) remain largely unexplored. METHODS: A-related enzymes. RESULTS: A methylation was mainly increased in lung tissues from MCT-PAH rats. The up-methylated coding genes in MCT-PAH rats were primarily enriched in processes associated with inflammation, glycolysis, ECM-receptor interaction and PDGF signal pathway, while genes with down-methylation were enriched in processes associated with TGF-β family receptor members. The expression of FTO and ALKBH5 downregulated, METTL3 and YTHDF1 increased and other methylation modification-related proteins was not significantly changed in MCT-PAH rats lung tissues. Immunofluorescence indicated that expression of FTO decreased and YTHDF1 increased in small pulmonary arteries of MCT-PAH rats. CONCLUSION: A modification.