The oxygen-resistant [FeFe]-hydrogenase CbA5H harbors an unknown radical signal
Melanie Heghmanns, Andreas Rutz, Yury Kutin, Vera Engelbrecht, Martin Winkler, Thomas Happe, Müge Kasanmascheff
Abstract
Fe enrichment combined with isotope-sensitive electron-nuclear double resonance (ENDOR) spectroscopy revealed a spin density distribution that resembles an H-cluster state. Overall, we uncovered a radical species in CbA5H that is potentially involved in the redox sensing of CbA5H.
Topics & Concepts
HydrogenaseElectron paramagnetic resonance spectroscopyElectron paramagnetic resonanceOxygenPhotochemistryChemistrySpectroscopyCluster (spacecraft)Nuclear magnetic resonanceCatalysisPhysicsBiochemistryComputer scienceOrganic chemistryQuantum mechanicsProgramming languageMetalloenzymes and iron-sulfur proteinsElectrocatalysts for Energy ConversionAmmonia Synthesis and Nitrogen Reduction