Coimmunoprecipitation with MYR1 Identifies Three Additional Proteins within the Toxoplasma gondii Parasitophorous Vacuole Required for Translocation of Dense Granule Effectors into Host Cells
Alicja M. Cygan, Terence C. Theisen, Alma G. Mendoza, Nicole D. Marino, Michael W. Panas, John C. Boothroyd
Abstract
Toxoplasma is an extremely successful intracellular parasite and important human pathogen. Upon infection of a new cell, Toxoplasma establishes a replicative vacuole and translocates parasite effectors across this vacuole to function from the host cytosol and nucleus. These effectors play a key role in parasite virulence. The work reported here newly identifies three parasite proteins that are necessary for protein translocation into the host cell. These results significantly increase our knowledge of the molecular players involved in protein translocation in Toxoplasma -infected cells and provide additional potential drug targets.
Topics & Concepts
EffectorImmunoprecipitationChromosomal translocationDense granuleCell biologyVacuoleToxoplasma gondiiCytosolBiologyCytoplasmSecretionTransport proteinIntracellularGranule (geology)AntibodyBiochemistryGeneticsGenePaleontologyEnzymeToxoplasma gondii Research StudiesMosquito-borne diseases and controlParasitic Infections and Diagnostics