Telomere length set point regulation in human pluripotent stem cells critically depends on the shelterin protein TPP1
John M. Boyle, Kelsey M. Hennick, Samuel G. Regalado, Jacob M. Vogan, Xiaozhu Zhang, Kathleen Collins, Dirk Hockemeyer
Abstract
To better understand telomere length set point control in human stem cells, we generated knockout stem cell lines for TPP1 and contrasted their phenotypes with those of homozygous TPP1 L104A mutant stem cells. This comparison reveals that TPP1 L104A is not a hypomorphic allele but formally establishes TPP1 L104 as a dissociation of function mutant.
Topics & Concepts
TelomereShelterinBiologyTelomeraseInduced pluripotent stem cellTelomere-binding proteinCell biologyStem cellPoint mutationMutantMolecular biologyGeneticsEmbryonic stem cellTranscription factorDNA-binding proteinDNAGeneTelomeres, Telomerase, and SenescenceRNA Interference and Gene DeliveryPluripotent Stem Cells Research